To do that, the inventors produced a significant plasmid (˜12 kb) exchanging the p15a-dependent origin of replication because of the primase origin. This plasmid targets the lacZ gene (p1322, SEQ ID NO: twenty five) as well as is made up of a chloramphenicol marker. because it was ignored In case the RBS toughness would want to generally be modifi… Read More

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